FASTERIS - Capillary Sequencing

Description

Prices

Requirements

Standard Primers

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Standard Primers

Supplied by FASTERIS free of charge.

Abbreviation	Name	Sequence
M13-Fwd	M13 Universal (-20)	5'-GTAAAACGACGGCCAGT-3'
M13-Rev	M13 Reverse (-24)	5'-AACAGCTATGACCATG-3'
T3	T3 Sequencing¹	5'-AATTAACCCTCACTAAAGG-3'
T7	T7 Sequencing¹	5'-TAATACGACTCACTATAGG-3'
SP6	SP6 Sequencing²	5'-GATTTAGGTGACACTATAG-3'
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N-CMV-30	N-CMV-30	5'-AATGTCGTAATAACCCCGCCCCGTTGACGC-3'
C-CMV-24	C-CMV-24	5'-TATTAGGACAAGGCTGGTGGGCAC-3'
CMV-IE	CMV-IE	5'-CGCAAATGGGCGGTAGGCGTG-3'
GFP-Seq	GFP Sequencing	5'-AGTTCGTGACCGCCGCCGGGA-3'
gt11-F	Lambda gt11 forward	5'-GGTGGCGACGACTCCTGGAGCCCG-3'
gt11-R	Lambda gt11 reverse	5'-TTGACACCAGACCAACTGGTAATG-3'
M13-Fwd(-40)	M13 Universal (-40)	5'-GTTTTCCCAGTCACGAC-3'
M13-Rev(-48)	M13 Reverse (-48)	5'-AGCGGATAACAATTTCACACAGGA-3'
M13-Seq	M13 Sequencing	5'-TCCCAGTCACGACGT-3'
pCI-Seq	pCI Sequencing	5'-AGTTCAATTACAGCTCTTAA-3'
pCI-Seq-Rev	pCI Reverse	5'-GCAATAGCATCACAAATTTCAC-3'
BGH-rev	BGH rev / pSecTag	5'-TAGAAGGCACAGTCGAGG-3'
PolyT-A	(T)23-A	5'-TTTTTTTTTTTTTTTTTTTTTTTA-3'
PolyT-C	(T)23-C	5'-TTTTTTTTTTTTTTTTTTTTTTTC-3'
PolyT-G	(T)23-G	5'-TTTTTTTTTTTTTTTTTTTTTTTG-3'
PolyT-V	(T)23-V	5'-TTTTTTTTTTTTTTTTTTTTTTTV-3'
SK primer	SK primer	5'-CTAGAACTAGTGGATCC-3'
T7-Term	T7 Terminator	5'-TAGTTATTGCTCAGCGGTGG-3'

(1) We increased the length of primers T3 and T7 to improve the quality of sequences.
(2) Formerly named New-SP6. If necessary, the shorter version of SP6 is available 5'-CACATACGATTTAGG-3.

Specific Primers

When supplying your own specific primer, please indicate its Tm and concentration.
It should be provided in a separate tube at 10 µM. We need approximately 1 µl per reaction.