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How to send your samples
Standard Service
Template DNA min. 5 uL per reaction in any tube
Primer at 10 uM in any tube
For more details, please refer to the Standard Protocol
One Shot or Economy
5 uL template DNA with 0.5 uL primer at 10 uM
Samples must be provided in 8, 48 or 96-wells plates
For more details, please refer to the OneShot & Economy Protocol
Ordering
First time users will need to register to get access to the DNA Sequencing Order Form.
The links for registration and ordering are found on the top left of our home page.
Place orders through our website.
Shipping
DNA templates and primers can be sent at room temperature.
Capillary Sequencing Service
Fasteris SA
Case postale 28
1228 Plan-les-Ouates
Switzerland
Tel: +41 22 794 22 42
sequencing(at)fasteris.com
By mail: use a bubble enveloppe. Free pre-paid A-post stickers available.
Pickup service in Geneva: place samples in a plastic bag on your lab's door. Orders are placed via our website. Please call when ordering after 12h30.
Please do not hesitate to contact us for testing our service: First 5 reactions offered.
Data delivery
Sequencing data is delivered by e-mail.
Upon request, large files are placed on our server for download
The sequences are saved under FASTA format in text files.
The chromatograms are saved under AB1 format compressed in a WinZIP archive.
Example of a control reaction, pGEM vector sequenced with the universal primer M13 Forward:
NB: the quality of the sequence depends significantly on the template and the primer (e.g. base composition, purification).
The chromatograms can be visualized using the following free programs:
- FinchTV (Mac OS X, Windows, Linux, Solaris), can show quality values
- 4Peaks (Mac OS X)), can show quality values
- Sequence Scanner Software (Windows), ABI's reader, can show quality values
- BioEdit (Windows)
- ApE- A Plasmid Editor (Mac OS X, Windows, Linux)
- Sequencher Demo (Mac OS 9 or X, Windows)
- Chromas LITE (Windows)
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