The Short Amplicon Sequencing approach is designed to sequence populations of amplicons ranging from 100 bp to 550 bp. It follows a two-step method:

Short amplicon (ligation of amplicons) is a Genesupport accredited ISO-17025:STS 0680 pipeline for Genesupport Life Science (Fasteris) NGS services.

Amplicon generation (metabarcoding)

Amplification of a specific region with tags, performed in your laboratories. The initial step involves a single PCR amplification using tagged primers (for sample identification), performed in your laboratory. Please note that the primers have specific requirements for the tag and the addition of random bases. Feel free to contact our team if you need further information.

Fasteris | Ligation of short amplicon protocol