The metabarcoding pipeline entails using PCR/barcode with next-generation sequencing approach to identify and to characterize species compositions within a sample or within complex population.

To this aim, Fasteris offers 2 alternative pipelines:

Metagenomics (16S/18S/ITS or custom PCR amplicons)

It is adapted for amplify target regions up to 550 bp amplicons and to sequence on the illumina machines.

Full length 16S rRNA sequencing

It aims to target and amplify the entire region of the 16S rRNA target gene and to be sequenced on the PacBio technology.

For both protocols, we perform a PCR amplification step to target the gene region (or regions) of interested, adding a specific barcode into the amplicons.

Region specific long amplicon sequencing protocol

This protocol allows to target and to amplify long specific genomic regions and to be then sequenced on PacBio technology. To reach this goal, we offer two independent protocols that differ from the costs and number of PCRs performed:

Region specific long amplicon with barcoded primers;
Region specific long amplicon with M13 approach.

Fasteris | Designed techniques for amplicon-based studies.