Transposon Sequencing (Tn-seq) is an in-house method for genome-wide profiling that allows for the accurate detection of transposon insertion sites following random mutagenesis. The protocol includes library preparation using the Twist kit, followed by the enrichment of inserts containing transposon sequences.

Our method enables the identification of transposon insertion sites after the enrichment step. The first 25 sequenced bases are from the transposon, resulting in a library with low diversity, which can impact sequencing quality. To address this issue, we add 20% PhiX to enhance diversity. This service is available only with full flow cell order.

During sequencing, the initial portion of the sequence corresponds to the end of the transposon (20-30 bp). To eliminate non-specific sequences, bioinformatic analysis first selects sequences containing the transposon, then removes this sequence to obtain the genomic insertion sequence.

Standard protocol: To view DNA samples specifications, sequencing recommendation and samples shipping guidelines, please download the corresponding SP-Doc.

SP-Doc / Tn-Seq

Fasteris | In-house designed protocol for Tn-Seq studies.