The Small RNA-Seq (Drosophila) protocol allows comprehensive analysis of the small non-coding RNA population, such as miRNA, siRNA and piRNA, in Drosophila samples.

The standard protocol consists of purification step for remove the mayor sRNA 2S subunit, following by library construction using Small RNA-Seq protocol.

This protocol requires as little as 100 ng of total RNA input. Thus, it can accommodate challenging samples. Use of Unique Molecular Indices (UMIs) tag eliminates PCR and sequencing bias.

Fasteris | Small-RNA-Seq protocol for fly samples