The following list summarizes the recommended input type and quantity for successfully library preparation service using the Illumina TruSeq Total stranded RNA (Ribo-Zero Gold/Globin/Plant) kit. Please contact our team if your samples do not belong to the listed categories:

Total RNA, 150-500 ng in 15 μl of RNase-free water (DNase treatment required)*.
Low amount total RNA, 15-100 ng in 15 μl of RNase-free water (DNase treatment required)*.

Samples concentration should be measured with fluorescence-based system.

Samples should possess an integrity value corresponding to RIN value >8 or equivalent otherwise a purity value corresponding to OD:260/230 >1.8 or equivalent.

However, for this protocol, we can accept also samples with less quality than our standard requirements.

Our standard protocol can be adjusted to individual needs by implementation of additional services.

*Includes additional material required to perform initial quality control.

RNA-seq studies with ribosomal RNA depletion.