Illumina platforms

The following list summarizes the recommended input type and quantity and all the necessary information to run successfully your libraries. Please contact our team if your samples do not belong to the listed categories.

Single library or pool of libraries, 5 nM in a minimal volume of 35 uL*.
Required information to be provided:
- Library or pool of libraries purified and prepared with illumina or illumina compatible kits
- Index sequences
- Index kind: single or dual
To ensure the right set of of your study and the quality of your samples, Fasteris performs, by default, a library profile on each tube received.

Library concentration should be measured with fluorescence-based system.

Please note that we will not be able to proceed with your project without having received the above information.

Fasteris will perform the entry QC by qPCR, but note that these data do not indicate or uncover use of false adapter versions, improper use of indexing and/or other errors that may happen when inexperienced person prepare illumina libraries the first time.

Ion Torrent platforms

The following list summarizes the recommended input type and quantity and all the necessary information to run successfully your libraries. Please contact our team if your samples do not belong to the listed categories:

Single library, 100 pM in in a minimal volume of 50 μL*.
Required information to be provided:
- Library prepared with ThermoFisher or ThermoFisher compatible kits
- Index sequences
- Run type
To ensure the right set of of your study and the quality of your samples, Fasteris performs, by default, a library profile on each tube received.

*Includes additional material required to perform initial quality control.

Ready-to-Run Sequencing