The specifications shown below (Typical run and Yield) come from the Illumina website for a full flow cell and the v1.5 reagent kits. Individual results may vary.

Exclusive sequencing package 800 M / Pass-filter clusters⁽¹⁾:

100 cycles

Typical run: 2x 50 bp
Output: 650-800 million clusters
Yield: 65 - 80 Gb

200 cycles

Typical run: 2x 100 bp
Output: 650-800 million clusters
Yield: 130 - 160 Gb

300 cycles

Typical run: 2x 150 bp
Output: 650-800 million clusters
Yield: 195 - 240 Gb

500 cycles

Typical run: 2x 250bp
Output: 650-800 million clusters
Yield: 325 - 400 Gb

Exclusive sequencing package 1600M / Pass-filter clusters⁽¹⁾:

100 cycles

Typical run: 2x 50 bp
Output: 1300-1600 million clusters
Yield: 130 - 160 Gb

200 cycles

Typical run: 2x 100 bp
Output: 1300-1600 million clusters
Yield: 260 - 320 Gb

300 cycles

Typical run: 2x 150 bp
Output: 1300-1600 million clusters
Yield: 390 - 480 Gb

Exclusive sequencing package 4100M / Pass-filter clusters⁽¹⁾:

100 cycles

Typical run: 2x 50 bp
Output: 3300-4100 million clusters
Yield: 330 - 410 Gb

200 cycles

Typical run: 2x 100 bp
3300-4100 million clusters
Yield: 660 - 820 Gb

300 cycles

Typical run: 2x 150 bp
3300-4100 million clusters
Yield: 990 - 1230 Gb

Exclusive sequencing package 10000M / Pass-filter clusters⁽¹⁾:

S4 Type 200 cycles

Typical run: 2x 100 bp
Output: 8000-10000 million clusters
Yield: 1.6 - 2.0 Tb

S4 Type 300 cycles

Typical run: 2x 150 bp
Output: 8000-10000 million clusters
Yield: 2.4 - 3.0 Tb

(1) Average yields are given by run information from Illumina. Not included are biased or non-standard samples. In sequencing runs with libraries exhibiting high genomic diversity, the maximum sequencing output is targeted. Conversely, for libraries with low genomic diversity, the minimum sequencing output is targeted. Furthermore, an additional percentage of PhiX, up to 30%, is incorporated into the run.

The final sequencing output of the flow cell, as well as for each individual library, may vary by up to 15% for libraries with high genomic diversity and by up to 20% for libraries with low genomic diversity.

Specifications and details are subject changing without notice.

Fasteris | Novaseq6000 technology